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Structured Review

Affinity Biosciences rabbit anti muc2 antibody
MFGE8 knockdown attenuates CSE-mediated restoration of the intestinal mucus barrier. ( A ) Representative Alcian blue-stained micrographs showing goblet cells (blue). Scale bar = 100 μm ( B ) Quantitative analysis of goblet cells in colon tissues. ( C ) <t>MUC2</t> immunofluorescence staining (red, muc2 marker) and DAPI (blue, nuclear staining) in colonic tissues. Scale bar = 100 μm. ( D ) Statistical analysis of the mean fluorescence intensity of MUC2. ( E ) Immunofluorescence staining of CD11c (green, dendritic cell marker) and DAPI (blue, nuclear staining) in colonic tissues. Scale bar = 100 μm. ( F ) Integral optical density quantification of CD11c staining. Data were analyzed using one-way ANOVA; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.
Rabbit Anti Muc2 Antibody, supplied by Affinity Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+muc2+antibody/pmc13022928-98-5-8?v=Affinity+Biosciences
Average 86 stars, based on 1 article reviews
rabbit anti muc2 antibody - by Bioz Stars, 2026-08
86/100 stars

Images

1) Product Images from "Oral Microcapsules Encapsulating Endometrial Regenerative Cell-Derived Exosomes Promote Intestinal Epithelial Barrier Repair and Ameliorate Experimental Colitis"

Article Title: Oral Microcapsules Encapsulating Endometrial Regenerative Cell-Derived Exosomes Promote Intestinal Epithelial Barrier Repair and Ameliorate Experimental Colitis

Journal: International Journal of Nanomedicine

doi: 10.2147/IJN.S540230

MFGE8 knockdown attenuates CSE-mediated restoration of the intestinal mucus barrier. ( A ) Representative Alcian blue-stained micrographs showing goblet cells (blue). Scale bar = 100 μm ( B ) Quantitative analysis of goblet cells in colon tissues. ( C ) MUC2 immunofluorescence staining (red, muc2 marker) and DAPI (blue, nuclear staining) in colonic tissues. Scale bar = 100 μm. ( D ) Statistical analysis of the mean fluorescence intensity of MUC2. ( E ) Immunofluorescence staining of CD11c (green, dendritic cell marker) and DAPI (blue, nuclear staining) in colonic tissues. Scale bar = 100 μm. ( F ) Integral optical density quantification of CD11c staining. Data were analyzed using one-way ANOVA; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.
Figure Legend Snippet: MFGE8 knockdown attenuates CSE-mediated restoration of the intestinal mucus barrier. ( A ) Representative Alcian blue-stained micrographs showing goblet cells (blue). Scale bar = 100 μm ( B ) Quantitative analysis of goblet cells in colon tissues. ( C ) MUC2 immunofluorescence staining (red, muc2 marker) and DAPI (blue, nuclear staining) in colonic tissues. Scale bar = 100 μm. ( D ) Statistical analysis of the mean fluorescence intensity of MUC2. ( E ) Immunofluorescence staining of CD11c (green, dendritic cell marker) and DAPI (blue, nuclear staining) in colonic tissues. Scale bar = 100 μm. ( F ) Integral optical density quantification of CD11c staining. Data were analyzed using one-way ANOVA; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Techniques Used: Knockdown, Staining, Immunofluorescence, Marker, Fluorescence



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MFGE8 knockdown attenuates CSE-mediated restoration of the intestinal mucus barrier. ( A ) Representative Alcian blue-stained micrographs showing goblet cells (blue). Scale bar = 100 μm ( B ) Quantitative analysis of goblet cells in colon tissues. ( C ) <t>MUC2</t> immunofluorescence staining (red, muc2 marker) and DAPI (blue, nuclear staining) in colonic tissues. Scale bar = 100 μm. ( D ) Statistical analysis of the mean fluorescence intensity of MUC2. ( E ) Immunofluorescence staining of CD11c (green, dendritic cell marker) and DAPI (blue, nuclear staining) in colonic tissues. Scale bar = 100 μm. ( F ) Integral optical density quantification of CD11c staining. Data were analyzed using one-way ANOVA; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.
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Image Search Results


MFGE8 knockdown attenuates CSE-mediated restoration of the intestinal mucus barrier. ( A ) Representative Alcian blue-stained micrographs showing goblet cells (blue). Scale bar = 100 μm ( B ) Quantitative analysis of goblet cells in colon tissues. ( C ) MUC2 immunofluorescence staining (red, muc2 marker) and DAPI (blue, nuclear staining) in colonic tissues. Scale bar = 100 μm. ( D ) Statistical analysis of the mean fluorescence intensity of MUC2. ( E ) Immunofluorescence staining of CD11c (green, dendritic cell marker) and DAPI (blue, nuclear staining) in colonic tissues. Scale bar = 100 μm. ( F ) Integral optical density quantification of CD11c staining. Data were analyzed using one-way ANOVA; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Journal: International Journal of Nanomedicine

Article Title: Oral Microcapsules Encapsulating Endometrial Regenerative Cell-Derived Exosomes Promote Intestinal Epithelial Barrier Repair and Ameliorate Experimental Colitis

doi: 10.2147/IJN.S540230

Figure Lengend Snippet: MFGE8 knockdown attenuates CSE-mediated restoration of the intestinal mucus barrier. ( A ) Representative Alcian blue-stained micrographs showing goblet cells (blue). Scale bar = 100 μm ( B ) Quantitative analysis of goblet cells in colon tissues. ( C ) MUC2 immunofluorescence staining (red, muc2 marker) and DAPI (blue, nuclear staining) in colonic tissues. Scale bar = 100 μm. ( D ) Statistical analysis of the mean fluorescence intensity of MUC2. ( E ) Immunofluorescence staining of CD11c (green, dendritic cell marker) and DAPI (blue, nuclear staining) in colonic tissues. Scale bar = 100 μm. ( F ) Integral optical density quantification of CD11c staining. Data were analyzed using one-way ANOVA; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Article Snippet: Immunostaining was performed using a rabbit anti-MUC2 antibody (Affinity Biosciences).

Techniques: Knockdown, Staining, Immunofluorescence, Marker, Fluorescence

(A) Expression of the mRNA levels of epithelial cell marker genes in IPEC-J2 and pig intestinal organoids. Data are presented as mean ± SD (n = 3–5). (B) Immunostaining of MUC2 in IPEC-J2 and pig intestinal organoids. (C) Immunostaining of CHGA in IPEC-J2 and pig intestinal organoids. Nucleus and F-actin were stained with Hoechst 33342 and phalloidin. Scale bar = 50 μm. * p < 0.05. ND, non-detected.

Journal: Journal of Animal Science and Technology

Article Title: Functional characteristics of a pig 2D intestinal organoid model as an in vitro platform for nutritional studies

doi: 10.5187/jast.2024.e116

Figure Lengend Snippet: (A) Expression of the mRNA levels of epithelial cell marker genes in IPEC-J2 and pig intestinal organoids. Data are presented as mean ± SD (n = 3–5). (B) Immunostaining of MUC2 in IPEC-J2 and pig intestinal organoids. (C) Immunostaining of CHGA in IPEC-J2 and pig intestinal organoids. Nucleus and F-actin were stained with Hoechst 33342 and phalloidin. Scale bar = 50 μm. * p < 0.05. ND, non-detected.

Article Snippet: The primary antibodies rabbit anti-Muc2 (27675-1-AP, ProteinTech) and mouse anti-ChgA (sc-393941, Santa Cruz Biotechnology) were diluted at 1:50 and 1:100, respectively, with the blocking buffer.

Techniques: Expressing, Marker, Immunostaining, Staining